Mouse anti Human IgG4 (pFc subclass specific)

Catalogue Number: MAHU/IGG4/MAB (1 MG)-NMB

Manufacturer:	Nordic MuBio
Type:	Monoclonal Secondary Antibody - Unconjugated
Shipping Condition:	RT
Unit(s):	1 mg
Host name:	Mouse
Clone:	NI 315 (HP 6206)
Isotype:	IgG1
Immunogen:
Application:	ELISA, ICC, IHC

Overview

Description

Description: The reactivity of the antiserum is restricted to a subclass specific determinant on the pFc portion of the IgG4 molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG4, and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.`

Additional Text

Antibody Clonality

Monoclonal

Application Notes

Immunohistochemistry, immunocytochemistry, ELISA

Storage Note

Lyophilized - can be stored at +4oC. After reconstitution aliquots can be stored at -20C. The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4C, not refrozen, and preferably used the same day.

Price

£1,715.00

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